CELSR1 Acts as an Oncogene Regulated by miR-199a-5p in Glioma

Guang Wang; Yong Li; Dongxia Zhang; Songtao Zhao; Qiong Zhang; Chao Luo; Xiaochuan Sun; Bingqian Zhang

doi:10.2147/CMAR.S258835

CELSR1 Acts as an Oncogene Regulated by miR-199a-5p in Glioma

Cancer Manag Res. 2020 Sep 23:12:8857-8865. doi: 10.2147/CMAR.S258835. eCollection 2020.

Authors

Guang Wang^{1

2}, Yong Li³, Dongxia Zhang⁴, Songtao Zhao⁴, Qiong Zhang⁴, Chao Luo², Xiaochuan Sun¹, Bingqian Zhang⁵

Affiliations

¹ Department of Neurosurgery, First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.
² Department of Neurosurgery, Chongqing Traditional Chinese Medicine Hospital, Chongqing, People's Republic of China.
³ Institute of Pathology and Southwest Cancer Center, Southwest Hospital Affiliated to Third Military Medical University, Chongqing, People's Republic of China.
⁴ National Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital Affiliated to Third Military Medical University, Chongqing, People's Republic of China.
⁵ Department of Clinical Medicine, Chongqing Engineering Research Center of Pharmaceutical Sciences, Chongqing Medical and Pharmaceutical College, Chongqing, People's Republic of China.

Abstract

Purpose: This study aimed to elucidate the biological function and upstream regulatory mechanism of CELSR1 in glioma.

Materials and methods: We evaluated the expression of CELSR1 in glioma by TCGA_GEPIA tool, RT-qPCR, and Western blot assays. CCK-8, wound healing, and transwell invasion assays were, respectively, performed to detect the effect of CELSR1 on cell proliferation, migration, and invasion. The upstream regulatory miRNAs of CELSR1 were predicted by TargetScan and validated by luciferase activity reporter assay.

Results: CELSR1 is overexpressed in glioma (P<0.05). CELSR1 promoted glioma cell proliferation, migration and invasion (P<0.01). CELSR1 was a direct target of miR-199a-5p. miR199a-5p mimics significantly inhibited CELSR1 mRNA and protein expression (P<0.01). miR199a-5p mimics reversed the effects of CELSR1 on glioma cell behaviors (P<0.01).

Conclusion: CELSR1 acts as an oncogene promoting glioma cell proliferation, migration, and invasion, which is regulated by miR199a-5p.

Keywords: CELSR1; glioma; invasion; miR-199a-5p; migration; proliferation.