MRI of [2-13 C]Lactate without J-coupling artifacts

Abstract

Purpose: Imaging of [2-¹³ C]lactate, a metabolic product of [2-¹³ C]pyruvate, is over considerable interest in hyperpolarized ¹³ C studies. However, artifact-free imaging of a J-coupled nuclear spin species can be challenging due to the peak-splitting induced by the spin-spin interactions. In this work, two new techniques resolving these J-modulated artifacts are presented.

Theory and methods: The Product Operator Formalism (POF) of density matrix theory is used to both numerically and analytically derive the coherences arising during radiofrequency excitation and readout of a J-coupled spin system. A combination of computer simulations and experiments with [2-¹³ C]lactate and ¹³ C-formate phantoms are then used to verify the performance of two imaging methods. In the first approach, a quadrature imaging technique is used to eliminate scalar coupling artifacts via the combination of in-phase and quadrature images acquired at echo times differing by 1/2J with an echoplanar readout. The second approach employs a highly narrowband RF excitation pulse to image a single peak from the J-coupled doublet.

Results: Simulations using a numerical Shepp-Logan phantom, in vitro experiments using thermally polarized [2-¹³ C]lactate, thermally and hyperpolarized ¹³ C-formate phantoms, and in vivo imaging of [2-¹³ C]lactate produced in rat brain following injection of hyperpolarized [2-¹³ C]pyruvate show artifact-free images and demonstrate potential utility of these methods.

Conclusion: The quadrature imaging and the narrowband excitation techniques resolve the J-coupling induced ghosting and blurring artifacts present with conventional MRI of J-coupled signals such as [2-¹³ C]lactate.

Keywords: J-coupling; [2-13C]lactate; [2-13C]pyruvate; hyperpolarized 13C metabolic imaging.