Viral metagenomic identification of a novel anellovirus in blood sample of a child with atopic dermatitis

J Med Virol. 2021 Jun;93(6):4038-4041. doi: 10.1002/jmv.26603. Epub 2020 Oct 30.

Abstract

Here, using viral metagenomics, a novel anellovirus with strain name HuAV-zj-ad1 was detected in blood sample from a child with atopic dermatitis. The complete genome sequence of HuAV-zj-ad1 was determined and fully characterized. The circular genome of HuAV-zj-ad1 is 2841 nt in length and includes four polyprotein ORFs. Phylogenetic analysis and pairwise sequence comparisons based on the amino acid sequences of ORF1, ORF2, ORF3, ORF4 indicated that HuAV-zj-ad1 belonged to a novel species within the genus Betatorquevirus. Polymerase chain reaction screening results showed this anellovirus was not present 50 blood samples from normal children. Whether this novel species of anellovirus has association with a certain disease needs further study.

Keywords: anellovirus; complete genome; new species; phylogenetic analysis; viral metagenomics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anelloviridae / classification
  • Anelloviridae / genetics*
  • Child, Preschool
  • Dermatitis, Atopic / complications
  • Dermatitis, Atopic / virology*
  • Genome, Viral*
  • Humans
  • Infant
  • Metagenomics / methods*
  • Phylogeny
  • RNA, Viral / genetics
  • Sequence Analysis, DNA
  • Virus Diseases / blood*
  • Virus Diseases / virology

Substances

  • RNA, Viral