Calomutilla panamensis Cambra, Brothers Quintero sp. nov., female and male from Panama and Venezuela, and the hitherto unknown male of Calomutilla crucigera (Burmeister, 1854), are described and illustrated. Calomutilla panamensis represents the northernmost distribution record of this Neotropical genus and the first record from Venezuela. A key to species, photographs of the types of most species, and a brief description about the mating behavior of C. panamensis are presented. The differentiation of Calomutilla and Pertyella Mickel, 1952 is discussed and illustrated for both sexes.
Keywords: Hymenoptera, Sphaeropthalminae, Pseudomethocini, mutillid wasp, taxonomy, Neotropical region.