Effects of Rht17 in combination with Vrn-B1 and Ppd-D1 alleles on agronomic traits in wheat in black earth and non-black earth regions

Pavel Yu Kroupin; Gennady I Karlov; Ludmila A Bespalova; Elena A Salina; Anastasiya G Chernook; Nobuyoshi Watanabe; Mikhail S Bazhenov; Vladimir V Panchenko; Lubov A Nazarova; Victor Ya Kovtunenko; Mikhail G Divashuk

doi:10.1186/s12870-020-02514-0

Effects of Rht17 in combination with Vrn-B1 and Ppd-D1 alleles on agronomic traits in wheat in black earth and non-black earth regions

BMC Plant Biol. 2020 Oct 14;20(Suppl 1):304. doi: 10.1186/s12870-020-02514-0.

Authors

Pavel Yu Kroupin^{1

2}, Gennady I Karlov^{1

2}, Ludmila A Bespalova³, Elena A Salina⁴, Anastasiya G Chernook^{1

2}, Nobuyoshi Watanabe⁵, Mikhail S Bazhenov^{1

2}, Vladimir V Panchenko³, Lubov A Nazarova⁶, Victor Ya Kovtunenko³, Mikhail G Divashuk^{7

8

9}

Affiliations

¹ Laboratory of Applied Genomics and Crop Breeding, All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya str. 42, Moscow, 127550, Russia.
² Centre for Molecular Biotechnology, Russian State Agrarian University-Moscow Timiryazev Agricultural Academy, Timiryazevskaya street, 49, Moscow, 127550, Russia.
³ Department of Breeding and Seed Production of Wheat and Triticale, National center of grain named after P.P. Lukyanenko, Central Estate of KNIISH, Krasnodar, 350012, Russia.
⁴ Kurchatov Genomics Center, Institute of Cytology and Genetics SB RAS, Prospekt Lavrentyeva 10, Novosibirsk, 630090, Russia.
⁵ College of Agriculture, Ibaraki University, 3-21-1 Chuo, Ami, Inashiki, Ibaraki, 300-0393, Japan.
⁶ Kurchatov Genomics Center-ARRIAB, All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya str. 42, Moscow, 127550, Russia.
⁷ Laboratory of Applied Genomics and Crop Breeding, All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya str. 42, Moscow, 127550, Russia. divashuk@gmail.com.
⁸ Centre for Molecular Biotechnology, Russian State Agrarian University-Moscow Timiryazev Agricultural Academy, Timiryazevskaya street, 49, Moscow, 127550, Russia. divashuk@gmail.com.
⁹ Kurchatov Genomics Center-ARRIAB, All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya str. 42, Moscow, 127550, Russia. divashuk@gmail.com.

Abstract

Background: Plant height is an important wheat trait that is regulated by multiple genes, among which Rht is of the utmost value. In wheat, Rht-B1p (=Rht17) is a mutant allele of the Rht gene that encodes for a DELLA-protein and results in the development of gibberellin-insensitive plants with a dwarfing phenotype. The pleiotropic effects of dwarfing genes on yield are highly dependent on both the genetic background and the environmental conditions. In Russia, the Central Non-Black Earth Region and Krasnodar Krai are two economically important regions that require differing management for sustainable wheat production for food, feed and industry. The purpose of our study was to compare the pleiotropic effects of Rht-B1p on the main valuable agronomic traits in the F_3:4 families of the spring bread wheat Chris Mutant/Novosibirskaya 67 in the genetic background of Vrn-B1a/vrn-B1 (spring/winter phenotype) and Ppd-D1a/Ppd-D1b (insensitivity/sensitivity to photoperiod) alleles in a field experiment in Moscow and Krasnodar Krai.

Results: Plant height was reduced on average by 21 cm (28%) and 25 cm (30%), respectively; Ppd-D1a slightly strengthened the dwarfing effect in Moscow and mitigated it in Krasnodar Krai. Grain weight of the main spike was reduced by Rht-B1p in Moscow and to lesser extent in Krasnodar; Ppd-D1a and Vrn-B1a tended to partially compensate for this loss in Krasnodar Krai. Thousand grain weight was reduced on average by 5.3 g (16%) and 2.9 g (10%) in Moscow and Krasnodar Krai, respectively, but was partially compensated for by Ppd-D1a in Krasnodar Krai. Harvest index was increased due to Rht-B1p by 6 and 10% in Moscow and Krasnodar Krai, respectively. Rht-B1p resulted in a delay of heading by 1-2 days in Moscow. Ppd-D1a accelerated heading by 1 day and 6 days in Moscow and in Krasnodar Krai, respectively.

Conclusions: Rht-B1p could be introduced into wheat breeding along with dwarfing genes such as Rht-B1b and Rht-D1b. Special attention should be paid to its combination with Ppd-D1a and Vrn-B1a as regulators of developmental rates, compensators of adverse effects of Rht-B1p on productivity and enhancers of positive effect of Rht-B1p on harvest index.

Keywords: Dwarfing genes; Harvest index; Photoperiod-response genes; Ppd; Rht; Vernalization genes; Vrn; Wheat (Triticum aestivum).

MeSH terms

Alleles
Genes, Plant*
Genetic Pleiotropy
MADS Domain Proteins / genetics*
Photoperiod
Plant Proteins / genetics*
Soil
Temperature
Triticum / genetics*
Triticum / growth & development

Substances

MADS Domain Proteins
Plant Proteins
RHT-D1A protein, Triticum aestivum
Soil