Effects of Peptide C12-OOWW-NH2 on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum

Xindan Li; Guirong Feng; Wenjun Wang; Lanhua Yi; Lili Deng; Kaifang Zeng

doi:10.3389/fmicb.2020.574882

Effects of Peptide C₁₂-OOWW-NH₂ on Transcriptome and Cell Wall of the Postharvest Fungal Pathogen Penicillium digitatum

Front Microbiol. 2020 Sep 17:11:574882. doi: 10.3389/fmicb.2020.574882. eCollection 2020.

Authors

Xindan Li¹, Guirong Feng¹, Wenjun Wang¹, Lanhua Yi^{1

2}, Lili Deng^{1

2}, Kaifang Zeng^{1

2}

Affiliations

¹ College of Food Science, Southwest University, Chongqing, China.
² Research Center of Food Storage & Logistics, Southwest University, Chongqing, China.

Abstract

In this study, the transcriptional profiling of Penicillium digitatum after C₁₂O₃TR treatment was analyzed by RNA-Seq technology. A total of 2562 and 667 genes in P. digitatum were differentially expressed after 2 and 12 h treatment, respectively. These genes were respectively mapped to 91 and 79 KEGG pathways. The expression patterns of differentially expressed genes (DEGs) at 2 and 12 h were similar, mainly were the metabolic processes in cell wall, cell membrane, genetic information and energy. Particularly, the main metabolic process which was affected by C₁₂O₃TR stress for 2 and 12 h was cell integrity, including cell wall and cell membrane. The changes of chitin in cell wall was observed by Calcofluor White (CFW) staining assay. The weaker blue fluorescence in the cell wall septa, the decrease of β-1, 3-glucan synthase activity and the increase of chitinase and AKP activity showed that C₁₂O₃TR could damage the cell wall integrity. In conclusion, these results suggested that C₁₂O₃TR could inhibit the growth of P. digitatum through various mechanisms at transcriptional level, and could influence the cell wall permeability and integrity.

Keywords: C12O3TR; Penicillium digitatum; RNA-Seq; cell wall; peptide.