Bovine in-vitro produced embryos: Development of embryo proper and associated membranes from day 26 to 47 of gestation

Hanne Skovsgaard Pedersen; Gianluca Mazzoni; Maria Pihl; Haja N Kadarmideen; Poul Hyttel; Henrik Callesen

doi:10.1016/j.repbio.2020.10.001

Bovine in-vitro produced embryos: Development of embryo proper and associated membranes from day 26 to 47 of gestation

Reprod Biol. 2020 Dec;20(4):595-599. doi: 10.1016/j.repbio.2020.10.001. Epub 2020 Oct 9.

Authors

Hanne Skovsgaard Pedersen¹, Gianluca Mazzoni², Maria Pihl³, Haja N Kadarmideen⁴, Poul Hyttel³, Henrik Callesen⁵

Affiliations

¹ Department of Animal Science, Aarhus University, Blichers Alle 20, DK-8830, Tjele, Denmark.
² Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 7, DK-1870, Frederiksberg C, Denmark; Department of Health Technology, Technical University of Denmark, Ørsteds Plads, Building 345C, DK-2800, Kgs. Lyngby, Denmark.
³ Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 7, DK-1870, Frederiksberg C, Denmark.
⁴ Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 7, DK-1870, Frederiksberg C, Denmark; Quantitative Genetics, Bioinformatics and Computational Biology Group, Department of Applied Mathematics and Computer Science, Technical University of Denmark, Kemitorvet, DK-2800, Kgs. Lyngby, Denmark.
⁵ Department of Animal Science, Aarhus University, Blichers Alle 20, DK-8830, Tjele, Denmark. Electronic address: henrik.callesen@anis.au.dk.

PMID: 33041222
DOI: 10.1016/j.repbio.2020.10.001

Abstract

Based on in-vitro produced (IVP) bovine embryos, embryo proper and embryonic/fetal membranes were studied in 12 pregnancies from day 26 to 47. The embryos/fetuses displayed external as well as internal development of organs and structures according to the expectations from comparable in-vivo studies. However, the embryonic/fetal membranes were shorter than those reported for in-vivo-derived embryos/fetuses on days 26-35 of calculated age, whereas on days 41-47 they were of comparable lengths.

Keywords: Cattle; Embryo; Growth; IVP; Placenta.

Publication types

Comparative Study

MeSH terms

Animals
Cattle / embryology*
Embryo Transfer / veterinary
Embryonic Development / physiology*
Extraembryonic Membranes / growth & development*
Female
Fertilization in Vitro / methods
Fertilization in Vitro / veterinary*
Gestational Age*
Pregnancy