A CRISPR-strategy for the generation of a detectable fluorescent hESC reporter line (WAe009-A-37) for the subpallial determinant GSX2

Dario Besusso; Andrea Cossu; Ayat Mohamed; Manuel Cernigoj; Paolo Codega; Maura Galimberti; Ilaria Campus; Paola Conforti; Elena Cattaneo

doi:10.1016/j.scr.2020.102016

A CRISPR-strategy for the generation of a detectable fluorescent hESC reporter line (WAe009-A-37) for the subpallial determinant GSX2

Stem Cell Res. 2020 Dec:49:102016. doi: 10.1016/j.scr.2020.102016. Epub 2020 Oct 1.

Authors

Dario Besusso¹, Andrea Cossu¹, Ayat Mohamed¹, Manuel Cernigoj¹, Paolo Codega¹, Maura Galimberti¹, Ilaria Campus¹, Paola Conforti¹, Elena Cattaneo¹

Affiliation

¹ Department of Biosciences, University of Milan, Milan 20122, Italy; Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi", Milan 20133, Italy.

PMID: 33039807
DOI: 10.1016/j.scr.2020.102016

Abstract

GSX2 is a homeobox transcription factor (TF) controlling the specification of the ventral lateral ganglionic eminence and its major derivative, the corpus striatum. Medium spiny neurons (MSNs) represent the largest cell component of the striatum and they are primarily affected in Huntington disease (HD). Here, we used CRISPR technology to generate a pluripotent GSX2-reporter human embryonic stem cell (hESC) line that can be leveraged to monitor striatal differentiation in real-time and to enrich for MSN-committed progenitors.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Clustered Regularly Interspaced Short Palindromic Repeats
Corpus Striatum
Embryonic Stem Cells
Human Embryonic Stem Cells*
Humans
Neurons