In this study, the artificial soil poisoning method was used to explore the antioxidative stress mechanism and gene changes of earthworms (Eisenia foetida) after application of nitenpyram. The toxic effects of nitenpyram on earthworms were combined with the method called the second-generation integrated biomarker response index method (IBRv2) to be comprehensively analyzed by studying the reactive oxygen species (ROS) content, superoxide dismutase (SOD) activity, catalase (CAT) activity, glutathione S-transferase (GST) activity, malondialdehyde (MDA) content and DNA damage degree in earthworms. The results showed that the ROS content in the high-concentration (2.5 mg/kg) nitenpyram treatment group changed significantly. The changes of antioxidant enzymes in earthworms were also obvious. In terms of SOD enzyme activity, under the induction of nitenpyram, SOD activity in the 1 mg/kg and 2.5 mg/kg treatment groups was significantly enhanced. The concentration-treated group could all affect the activity of earthworm detoxifying enzyme GST. Earthworm DNA olive tail in the nitenpyram treatment group with different concentrations was mainly concentrated at low and medium levels at 21d, and the proportion was the largest during the whole exposure period, showing a significant dose-effect relationship. This study confirms that nitenpyram not only has a toxic effect on the physiological and biochemical indicators of earthworms, but also cannot be underestimated on its genetic level.
Keywords: Comet assay; Earthworms; Nitenpyram; Oxidative stress.
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