Generation of an integration-free induced pluripotent stem cell line (UNC001-A) from blood of a healthy individual

Sarahi G Molina; Alvaro A Beltran; Adriana S Beltran

doi:10.1016/j.scr.2020.102015

Generation of an integration-free induced pluripotent stem cell line (UNC001-A) from blood of a healthy individual

Stem Cell Res. 2020 Dec:49:102015. doi: 10.1016/j.scr.2020.102015. Epub 2020 Oct 1.

Authors

Sarahi G Molina¹, Alvaro A Beltran², Adriana S Beltran³

Affiliations

¹ Human Pluripotent Stem Cell Core, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States.
² Department of Neuroscience, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States.
³ Human Pluripotent Stem Cell Core, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States; Department of Pharmacology at the University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States. Electronic address: beltran@med.unc.edu.

PMID: 33038744
DOI: 10.1016/j.scr.2020.102015

Abstract

Induced pluripotent stem cells (iPSCs) generated from young, healthy individuals are valuable tools for investigating molecular disease mechanisms during the early development of the brain vasculature. We generated an iPSC line from peripheral blood mononuclear cells (PBMCs) isolated from a healthy 13-yeard old female donor using the Sendai virus. The iPSCs differentiated into endothelial cells, astrocytes, and neurons. This iPSC line can serve as a healthy reference control for comparative studies in drug development and modeling the early onset of Cerebral Cavernous Malformation (CCM).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Endothelial Cells
Female
Humans
Induced Pluripotent Stem Cells*
Leukocytes, Mononuclear
Sendai virus / genetics