Prospects of next generation sequencing in lentil breeding

Mol Biol Rep. 2020 Nov;47(11):9043-9053. doi: 10.1007/s11033-020-05891-9. Epub 2020 Oct 10.

Abstract

Lentil is an important food legume crop that has large and complex genome. During past years, considerable attention has been given on the use of next generation sequencing for enriching the genomic resources including identification of SSR and SNP markers, development of unigenes, transcripts, and identification of candidate genes for biotic and abiotic stresses, analysis of genetic diversity and identification of genes/ QTLs for agronomically important traits. However, in other crops including pulses, next generation sequencing has revolutionized the genomic research and helped in genomic assisted breeding rapidly and cost effectively. The present review discuss current status and future prospects of the use NGS based breeding in lentil.

Keywords: DNA sequencing; Functional markers; Genetic gain; Lentil; Pan-genome; Transcriptome; Unigenes.

Publication types

  • Review

MeSH terms

  • Crops, Agricultural / genetics*
  • Gene Expression Profiling / methods
  • Gene Expression Regulation, Plant
  • Genes, Plant / genetics*
  • Genomics / methods
  • High-Throughput Nucleotide Sequencing / methods*
  • Lens Plant / genetics*
  • Plant Breeding / methods*
  • Polymorphism, Single Nucleotide*
  • Quantitative Trait Loci / genetics