The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans

Abstract

Six CO₂ fixation pathways are known to operate in photoautotrophic and chemoautotrophic microorganisms. Here, we describe chemolithoautotrophic growth of the sulphate-reducing bacterium Desulfovibrio desulfuricans (strain G11) with hydrogen and sulphate as energy substrates. Genomic, transcriptomic, proteomic and metabolomic analyses reveal that D. desulfuricans assimilates CO₂ via the reductive glycine pathway, a seventh CO₂ fixation pathway. In this pathway, CO₂ is first reduced to formate, which is reduced and condensed with a second CO₂ to generate glycine. Glycine is further reduced in D. desulfuricans by glycine reductase to acetyl-P, and then to acetyl-CoA, which is condensed with another CO₂ to form pyruvate. Ammonia is involved in the operation of the pathway, which is reflected in the dependence of the autotrophic growth rate on the ammonia concentration. Our study demonstrates microbial autotrophic growth fully supported by this highly ATP-efficient CO₂ fixation pathway.