Purification of Yeast Spores to Investigate Their Dynamics of Activation

Curr Protoc Microbiol. 2020 Dec;59(1):e123. doi: 10.1002/cpmc.123.

Abstract

Germination is an important developmental process that supports resumption of growth in dormant spores. The study of the mechanisms underlying germination requires a pure spore population devoid of other cell types. This article describes the sporulation of wild Saccharomyces cerevisiae and Saccharomyces paradoxus strains, and the isolation and purification of ascospores. We also describe a method to synchronously induce germination in a spore population as well as to measure spore activation. This procedure can be applied, for example, to the study of environmental conditions that trigger germination. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Sporulation Basic Protocol 2: Spore purification Basic Protocol 3: Germination induction Support Protocol 1: Flow cytometry analysis Support Protocol 2: Heat-shock resistance measurement.

Keywords: Saccharomyces purification of yeast spores to investigate their dynamic of activation; germination; heat resistance; spore purification; sporulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Flow Cytometry
  • Heat-Shock Response
  • Hot Temperature
  • Microbiological Techniques / methods*
  • Saccharomyces
  • Saccharomyces cerevisiae
  • Spores, Fungal / physiology*
  • Yeasts / isolation & purification*

Supplementary concepts

  • Saccharomyces paradoxus