Overcoming matrix effects in the analysis of pyrethroids in honey by a fully automated direct immersion solid-phase microextraction method using a matrix-compatible fiber

João Raul Belinato; Jonathan J Grandy; Abir Khaled; Paola Alejandra Ortiz Suarez; Janusz Pawliszyn

doi:10.1016/j.foodchem.2020.128127

Overcoming matrix effects in the analysis of pyrethroids in honey by a fully automated direct immersion solid-phase microextraction method using a matrix-compatible fiber

Food Chem. 2021 Mar 15:340:128127. doi: 10.1016/j.foodchem.2020.128127. Epub 2020 Sep 24.

Authors

João Raul Belinato¹, Jonathan J Grandy², Abir Khaled², Paola Alejandra Ortiz Suarez², Janusz Pawliszyn³

Affiliations

¹ Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada; Institute of Chemistry, University of Campinas, Campinas, São Paulo 13083-970, Brazil and National Institute of Science and Technology in Bioanalysis (INCTBio).
² Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada.
³ Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada. Electronic address: janusz@uwaterloo.ca.

PMID: 33032149
DOI: 10.1016/j.foodchem.2020.128127

Abstract

Pyrethroids insecticides may constitute a major hazard to honeybees, leading to colony collapse disorder. However, the determination of pyrethroids in honey has remained a challenging undertaking for analysts to date due to the high complexity of this matrix as well as the MRLs. This paper presents a fully automated method to overcome matrix influences using matrix-compatible overcoated SPME fiber for quantitative analysis of pyrethroids in diluted honey by GC-MS. The developed method was optimized using a multivariate approach providing LOQ values much lower than the stablished MRL (0.10-10 ng/g), while granting satisfactory linearity (R² > 0.998) in a wide linear range of 0.1-2000 ng/g, repeatability with RSDs < 10%, reproducibility RSDs < 20%, and accuracy ranging from 75 to 118% and from 82 to 120 % for inter-day and intra-day assays, respectively by using five replicates. The method herein proposed overcomes challenges presented by complex matrices while minimizing sample handling and the overall complexity of the procedure.

Keywords: Automated; GC–MS; Honey; Matrix-compatible; Pyrethroids; Solid-phase microextraction.

MeSH terms

Automation
Gas Chromatography-Mass Spectrometry
Honey / analysis*
Limit of Detection
Pyrethrins / analysis*
Pyrethrins / isolation & purification
Reproducibility of Results
Solid Phase Microextraction / methods*

Substances

Pyrethrins