Reduction of the Carbapenemase Inactivation Method (CIM) assay time by real-time PCR

F Haque; S Fisseha; P Athamanolap; R Tower; J Ortega; C Dominguez; T Maruca; D Torpey; R Myers; P Laksanalamai

doi:10.1016/j.mimet.2020.106072

Reduction of the Carbapenemase Inactivation Method (CIM) assay time by real-time PCR

J Microbiol Methods. 2020 Oct 6:178:106072. doi: 10.1016/j.mimet.2020.106072. Online ahead of print.

Authors

F Haque¹, S Fisseha¹, P Athamanolap², R Tower¹, J Ortega¹, C Dominguez¹, T Maruca¹, D Torpey¹, R Myers¹, P Laksanalamai³

Affiliations

¹ Maryland Department of Health, Laboratories Administration, 1770 Ashland Ave., Baltimore, MD 21205, United States of America.
² Department of Biomedical Engineering, Faculty of Engineering, Mahidol University, 999 Phuttamonthon4 Road, Salaya, Nakhon Pathom 73170, Thailand.
³ Maryland Department of Health, Laboratories Administration, 1770 Ashland Ave., Baltimore, MD 21205, United States of America. Electronic address: pongpan.laksanalamai@maryland.gov.

PMID: 33031896
DOI: 10.1016/j.mimet.2020.106072

Abstract

Carbapenemase Inactivation Method (CIM) is a test to detect presence of the carbapenemase in Gram-negative bacteria. Determination of the carbapenemase production by inactivation of meropenem requires that a zone of control E. coli inhibition be measured approximately 6-24 h after plating. We have modified the CIM test by developing a rapid method which instead measures the growth of E. coli indicator strain ATCC 25922 using real-time PCR, referred to as a nucleic acid testing CIM (natCIM). Our natCIM, therefore reduces the detecting time from 6 to 24 h to approximately 4 h.

Keywords: Antimicrobial resistance; Carbapenemase Inactivation Method (CIM); PCR; natCIM.