Chemically modified mRNA nucleofection of primary human T cells

Abstract

Here we show that an approach of in-vitro transcribed mRNA nucleofection expands the range of transfection of primary human T cells. It represents a reproducible and time-efficient technology, and is thus an ideal tool in basic research involving highly controlled in-vitro experiments with a gene of interest aiming at identifying its biological human T cell function.

Keywords: In-vitro transcribed mRNA; NR2F6; Nucleofection; Primary human T cell.