Structures from the Mesophase: MicroED Targets Crystals Extracted from LCP

Marcus Gallagher-Jones; Jose A Rodriguez

doi:10.1016/j.str.2020.09.005

Structures from the Mesophase: MicroED Targets Crystals Extracted from LCP

Structure. 2020 Oct 6;28(10):1084-1086. doi: 10.1016/j.str.2020.09.005.

Authors

Marcus Gallagher-Jones¹, Jose A Rodriguez²

Affiliations

¹ Department of Chemistry and Biochemistry, UCLA-DOE Institute for Genomics and Proteomics, STROBE, NSF Science and Technology Center, University of California, Los Angeles, Los Angeles, CA 90095, USA.
² Department of Chemistry and Biochemistry, UCLA-DOE Institute for Genomics and Proteomics, STROBE, NSF Science and Technology Center, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: jrodriguez@mbi.ucla.edu.

PMID: 33027690
DOI: 10.1016/j.str.2020.09.005

Abstract

The determination of protein structures from nanocrystals grown in lipidic cubic phase (LCP) is a promising crystallographic approach. In this issue of Structure, Zhu et al. (2020) extract crystals from the dense matrix of monoolein LCP for interrogation by micro electron diffraction (MicroED) and yield a 2 Å structure of Proteinase K.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Comment

MeSH terms

Crystallography
Electrons*
Lipids*
Proteins

Substances

Lipids
Proteins

Grants and funding

R35 GM128867/GM/NIGMS NIH HHS/United States