Inter-laboratory comparison of 2 ELISA kits used for foot-and-mouth disease virus nonstructural protein serology

J Vet Diagn Invest. 2020 Nov;32(6):933-937. doi: 10.1177/1040638720962070. Epub 2020 Oct 7.

Abstract

Serologic assays used to detect antibodies to nonstructural proteins (NSPs) of foot-and-mouth disease virus (FMDV) are used for disease surveillance in endemic countries, and are essential to providing evidence for freedom of the disease with or without vaccination and to recover the free status of a country after an outbreak. In a 5-site inter-laboratory study, we compared the performance of 2 commercial NSP ELISA kits (ID Screen FMD NSP ELISA single day [short] and overnight protocols, ID.Vet; PrioCHECK FMDV NS antibody ELISA, Thermo Fisher Scientific). The overall concordance between the PrioCHECK and ID Screen test was 93.8% (95% CI: 92.0-95.2%) and 94.8% (95% CI: 93.1-96.1%) for the overnight and short ID Screen incubation protocols, respectively. Our results indicate that the assays (including the 2 different formats of the ID Screen test) can be used interchangeably in post-outbreak serosurveillance.

Keywords: ELISA; foot-and-mouth disease virus; inter-laboratory study; serology.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antibodies, Viral / blood
  • Cattle
  • Cattle Diseases / blood
  • Cattle Diseases / diagnosis*
  • Cattle Diseases / virology
  • Enzyme-Linked Immunosorbent Assay / standards
  • Enzyme-Linked Immunosorbent Assay / veterinary*
  • Foot-and-Mouth Disease / blood
  • Foot-and-Mouth Disease / diagnosis*
  • Foot-and-Mouth Disease / virology
  • Foot-and-Mouth Disease Virus
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Viral Nonstructural Proteins / metabolism*

Substances

  • Antibodies, Viral
  • Viral Nonstructural Proteins