In vivo clonal analysis of aging hematopoietic stem cells

Mech Ageing Dev. 2020 Dec:192:111378. doi: 10.1016/j.mad.2020.111378. Epub 2020 Oct 3.

Abstract

Hematopoietic stem cells (HSCs) are characterized by two key features: Self-renewal ability and multilineage differentiation potential (multipotentiality). With aging, these key features gradually change. This is thought to be related to hematological diseases. However, clonal in vivo analysis assessing the potential of HSCs to differentiate along erythroid and platelet lineages ("five-lineage tracing") has not been performed in the aged bone marrow. By contrast, in young HSCs clonal in vivo analysis combined with five-lineage tracing has provided us with novel insights into HSC biology. Understanding HSC aging at the clonal level will help us to elucidate aging mechanisms and disease progression. We review recent progress towards understanding HSC aging at the clonal cell level in the transplantation setting.

Keywords: Five-lineage tracing; Hematopoietic stem cell aging; Latent hematopoietic stem cell; Myeloid-restricted repopulating progenitor; Myeloid-restricted stem cell.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Animals
  • Cell Differentiation*
  • Cell Lineage
  • Cell Self Renewal / physiology
  • Cell Transdifferentiation*
  • Cellular Senescence / physiology*
  • Clonal Hematopoiesis / physiology*
  • Colony-Forming Units Assay
  • Hematopoietic Stem Cells / physiology*
  • Humans
  • Myeloid Progenitor Cells / physiology*