Biocompatible Crosslinked Nanofibers of Poly(Vinyl Alcohol)/Carboxymethyl-Kappa-Carrageenan Produced by a Green Process

Liszt Y C Madruga; Rosangela C Balaban; Ketul C Popat; Matt J Kipper

doi:10.1002/mabi.202000292

Biocompatible Crosslinked Nanofibers of Poly(Vinyl Alcohol)/Carboxymethyl-Kappa-Carrageenan Produced by a Green Process

Macromol Biosci. 2021 Jan;21(1):e2000292. doi: 10.1002/mabi.202000292. Epub 2020 Oct 5.

Authors

Liszt Y C Madruga^{1

2}, Rosangela C Balaban¹, Ketul C Popat^{3

4

5}, Matt J Kipper^{2

3

4}

Affiliations

¹ Institute of Chemistry, Federal University of Rio Grande do Norte (UFRN), Natal, RN, 59078-970, Brazil.
² Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO, 80523, USA.
³ School of Advanced Materials Discovery, Colorado State University, Fort Collins, CO, 80523, USA.
⁴ School of Biomedical Engineering, Colorado State University, Fort Collins, CO, 80523, USA.
⁵ Department of Mechanical Engineering, Colorado State University, Fort Collins, CO, 80523, USA.

PMID: 33021064
DOI: 10.1002/mabi.202000292

Abstract

This study presents a new type of biocompatible nanofiber based on poly(vinyl alcohol) (PVA) and carboxymethyl-kappa-carrageenan (CMKC) blends, produced with no generation of hazardous waste. The nanofibers are produced by electrospinning using PVA:CMKC blends with ratios of 1:0, 1:0.25, 1:0.4, 1:0.5, and 1:0.75 (w/w PVA:CMKC) in aqueous solution, followed by thermal crosslinking. The diameter of the fibers is in the nanometer scale and below 300 nm. Fourier transform infrared spectroscopy shows the presence of the carboxyl and sulfate groups in all the fibers with CMKC. The nanofibers from water-soluble polymers are stabilized by thermal crosslinking. The incorporation of CMKC improves cytocompatibility, biodegradability, cell growth, and cell adhesion, compared to PVA nanofibers. Furthermore, the incorporation of CMKC modulates phenotype of human adipose-derived stem cells (ADSCs). PVA/CMKC nanofibers enhance ADSC response to osteogenic differentiation signals and are therefore good candidates for application in tissue engineering to support stem cells.

Keywords: carrageenan; electrospinning; green chemistry; polymer blend; tissue engineering.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biocompatible Materials / chemical synthesis
Biocompatible Materials / chemistry
Biocompatible Materials / pharmacology*
Carrageenan / chemistry
Carrageenan / pharmacology
Cell Adhesion / drug effects
Cell Proliferation / drug effects
Fibroblasts / drug effects
Humans
Microscopy, Electron, Scanning
Nanofibers / chemistry*
Osteogenesis / drug effects*
Polyvinyl Alcohol / chemistry
Polyvinyl Alcohol / pharmacology
Spectroscopy, Fourier Transform Infrared
Tissue Engineering*
Tissue Scaffolds / chemistry

Substances

Biocompatible Materials
Carrageenan
Polyvinyl Alcohol