Model-based Analysis for Real-time Label-free Ultraviolet Quantification of Ultrafast Plasmonic Polymerase Chain Reaction

P Mohammadyousef; M Paliouras; M Trifiro; A G Kirk

doi:10.1109/EMBC44109.2020.9176669

Model-based Analysis for Real-time Label-free Ultraviolet Quantification of Ultrafast Plasmonic Polymerase Chain Reaction

Annu Int Conf IEEE Eng Med Biol Soc. 2020 Jul:2020:5136-5139. doi: 10.1109/EMBC44109.2020.9176669.

Authors

P Mohammadyousef, M Paliouras, M Trifiro, A G Kirk

PMID: 33019142
DOI: 10.1109/EMBC44109.2020.9176669

Abstract

A mathematical model for DNA quantification was calibrated using experimental results from real-time 260nm absorption measurements of plasmonic PCR thermocycling. The effect of different PCR parameters on template amplification was investigated using the calibrated model.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA*
Diagnostic Tests, Routine*
Polymerase Chain Reaction

Substances

DNA