This paper evaluated the pupillary light reflex of glaucomatous eyes in the presence of constant lighting via light-induced pupillometry using sample entropy. The study used 20 patients and 15 controls, applied three different light intensities to their eyes, and recorded the behavior of the pupil. This study has validated that there is a difference in the entropy of pupillary data in glaucoma and healthy eyes. We concluded that entropy analysis is an excellent method to differentiate glaucoma eyes with the control through light-induced pupillometry. Hence, pupillometry has potential clinical applications in glaucoma investigation.