Diagnostic approaches and contribution of next-generation sequencing technologies in genomic investigation of Vibrio parahaemolyticus that caused acute hepatopancreatic necrosis disease (AHPND)

Lean Huat Yu; Cindy Shuan Ju Teh; Kien Pong Yap; Kwai Lin Thong

doi:10.1007/s10499-020-00610-4

Diagnostic approaches and contribution of next-generation sequencing technologies in genomic investigation of Vibrio parahaemolyticus that caused acute hepatopancreatic necrosis disease (AHPND)

Aquac Int. 2020;28(6):2547-2559. doi: 10.1007/s10499-020-00610-4. Epub 2020 Sep 26.

Authors

Lean Huat Yu¹, Cindy Shuan Ju Teh², Kien Pong Yap¹, Kwai Lin Thong³

Affiliations

¹ Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
² Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
³ Nanocat Research Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia.

Abstract

A unique strain of Vibrio parahaemolyticus (designated as VP_AHPND) causes acute hepatopancreatic necrosis disease (AHPND), a deadly bacterial disease associated with mass mortality in cultured shrimps since 2009. AHPND is responsible for severe economic losses worldwide, causing multimillion-dollar loss annually. Because of the rapid and high mortality rates in shrimps, substantial research has been carried out to develop rapid detection techniques. Also, recent technological advances such as the next-generation sequencing (NGS) have made it possible to elucidate relevant information about a pathogen in a single assay. This review summarizes the current research pertaining to VP_AHPND, focusing on diagnosis and contribution of NGS technologies in the genomic studies of AHPND.

Keywords: Acute hepatopancreatic necrosis disease; Diagnostics; Next-generation sequencing; Vibrio parahaemolyticus.

Publication types

Review