Dinuclear copper(II) complexes with derivative triazine ligands as biomimetic models for catechol oxidases and nucleases

Abstract

The research reported herein focuses on the synthesis of two new Cu(II) complexes {[Cu₂(2-X-4,6-bis(di-2-picolylamino)-1,3,5-triazine], with X = butane-1,4-diamine (2) or N-methylpyrenylbutane-1,4-diamine (3)}, the latter with a pyrene group as a possible DNA intercalating agent. The structure of complex (3) was determined by X-ray crystallography and shows the dinuclear {Cu^II(μ-OCH₃)₂Cu^II} unit in which the Cu^II···Cu^II distance of 3.040 Å is similar to that of 2.97 Å previously found for 1, which contains a {Cu^II(μ-OH)₂Cu^II} structural unit. Complexes (2) and (3) were also characterized in spectroscopic and electrochemical studies, and catecholase-like activity were performed for both complexes. The kinetic parameters obtained for the oxidation of the model substrate 3,5-di-tert-butylcatechol revealed that the insertion of the spacer butane-1,4-diamine and the pyrene group strongly contributes to increasing the catalytic efficiency of these systems. In fact, K_ass becomes significantly higher, indicating that these groups influence the interaction between the complex and the substrate. These complexes also show DNA cleavage under mild conditions with moderate reaction times. The rate of cleavage (k_cat) indicated that the presence of butane-1,4-diamine and pyrene increased the activity of both complexes. The reaction mechanism seems to have oxidative and hydrolytic features and the effect of DNA groove binding compounds and circular dichroism indicate that all complexes interact with plasmid DNA through the minor groove. High-resolution DNA cleavage assays provide information on the interaction mechanism and for complex (2) a specificity for the unpaired hairpin region containing thymine bases was observed, in contrast to (3).

Keywords: Artificial nucleases; Catecholase activity; Copper complexes; DNA cleavage; Second coordination sphere; π-Stacking interaction.