A ratiometric electrochemiluminescence resonance energy transfer platform based on novel dye BODIPY derivatives for sensitive detection of lactoferrin

Yao Lu; Hong Ke; Yang Wang; Yao Zhang; Huan Li; Chusen Huang; Nengqin Jia

doi:10.1016/j.bios.2020.112664

A ratiometric electrochemiluminescence resonance energy transfer platform based on novel dye BODIPY derivatives for sensitive detection of lactoferrin

Biosens Bioelectron. 2020 Dec 15:170:112664. doi: 10.1016/j.bios.2020.112664. Epub 2020 Sep 28.

Authors

Yao Lu¹, Hong Ke¹, Yang Wang¹, Yao Zhang¹, Huan Li¹, Chusen Huang¹, Nengqin Jia²

Affiliations

¹ The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, College of Chemistry and Materials Science, Shanghai Normal University, Shanghai, 200234, PR China.
² The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, College of Chemistry and Materials Science, Shanghai Normal University, Shanghai, 200234, PR China. Electronic address: nqjia@shnu.edu.cn.

PMID: 33011620
DOI: 10.1016/j.bios.2020.112664

Abstract

A ratiometric electrochemiluminescence resonance energy transfer (ECL-RET) platform depended on novel dye BODIPY derivatives was proposed for rapid detection of lactoferrin. This ECL-RET platform is composed of aptamer decorated BODIPY composites and C₆₀@BSA, in which BODIPY derivative is the ECL probe and can generate significant resonance energy transfer with K₂S₂O₈. BODIPY derivative and K₂S₂O₈ are used as built-in reference signal and calibration signal respectively to eliminate background signal and abnormal change signal by double signal self-calibration process. At the same time, C₆₀, as the accelerator of K₂S₂O₈, can effectively increase the ECL signal and further transfer as much energy as possible to BODIPY derivative. Under optimal conditions, the constructed ECL-RET platform exhibited sensitive detection of lactoferrin in the wide linear range of 10^-4- 850 ng/mL with a LOD of 42 fg/mL. Meanwhile, the proposed ECL-RET aptasensor demonstrated superior stability, specificity and reproducibility, displaying favorable application value in practical diagnosis of this method.

Keywords: BODIPY derivative; Lactoferrin; Ratiometric electrochemiluminescence resonance energy transfer.

MeSH terms

Biosensing Techniques*
Boron Compounds
Electrochemical Techniques*
Energy Transfer
Lactoferrin
Luminescent Measurements
Reproducibility of Results

Substances

4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
Boron Compounds
Lactoferrin