The Evolving Role of the Clinical Microbiology Laboratory in Identifying Resistance in Gram-Negative Bacteria: An Update

Infect Dis Clin North Am. 2020 Dec;34(4):659-676. doi: 10.1016/j.idc.2020.08.001. Epub 2020 Sep 30.

Abstract

The evolution of resistance to antimicrobial agents in gram-negatives has challenged the role of the clinical microbiology laboratory to implement new methods for their timely detection. Recent development has enabled the use of novel methods for more rapid pathogen identification, antimicrobial susceptibility testing, and detection of resistance markers. Commonly used methods improve the rapidity of resistance detection from both cultured bacteria and specimens. This review focuses on the commercially available systems available together with their technical performance and possible clinical impact.

Keywords: AST; Blood; LAMP; MALDI-TOF; PCR; Rapid; Sequencing; T2MR.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / genetics
  • Bacterial Typing Techniques
  • Drug Resistance, Bacterial*
  • Gram-Negative Bacteria / classification
  • Gram-Negative Bacteria / drug effects
  • Gram-Negative Bacteria / isolation & purification*
  • Humans
  • Laboratories
  • Microbial Sensitivity Tests
  • Microbiological Techniques / methods*
  • Nucleic Acid Amplification Techniques
  • Whole Genome Sequencing

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins