Abstract
A highly oxygenated phenethyl derivative ustethylin A was isolated from Aspergillus ustus. Gene deletion, isotope labeling, and heterologous expression proved that the phenethyl core structure is assembled from malonyl-CoA by a polyketide synthase harboring a methyltransferase domain. Propionate was converted via acetyl-CoA to malonyl-CoA and incorporated into the molecule. Modifications on the core structure by three different oxidoreductases and one O-methyltransferase lead to the final product, ustethylin A.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetyl Coenzyme A / chemistry*
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Acetyl Coenzyme A / metabolism
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Aspergillus / chemistry*
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Malonyl Coenzyme A / chemistry*
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Malonyl Coenzyme A / isolation & purification
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Malonyl Coenzyme A / metabolism
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Methyltransferases / chemistry
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Methyltransferases / metabolism*
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Molecular Structure
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Oxidoreductases / chemistry
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Oxidoreductases / metabolism*
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Polyketide Synthases / chemistry
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Polyketide Synthases / metabolism*
Substances
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Malonyl Coenzyme A
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Acetyl Coenzyme A
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Polyketide Synthases
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Oxidoreductases
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Methyltransferases