Development of methods for detecting the fate of mesenchymal stem cells regulated by bone bioactive materials

Jiang Le; Liu Zhongqun; Wang Zhaoyan; Su Yijun; Wang Yingjin; Wei Yaojie; Jiang Yanan; Jia Zhanrong; Ma Chunyang; Gang Fangli; Xu Nan; Zhao Lingyun; Wang Xiumei; Wu Qiong; Lu Xiong; Sun Xiaodan

doi:10.1016/j.bioactmat.2020.08.035

Development of methods for detecting the fate of mesenchymal stem cells regulated by bone bioactive materials

Bioact Mater. 2020 Sep 19;6(3):613-626. doi: 10.1016/j.bioactmat.2020.08.035. eCollection 2021 Mar.

Authors

Jiang Le^{1

2}, Liu Zhongqun^{1

2}, Wang Zhaoyan^{3

4

5}, Su Yijun^{1

2}, Wang Yingjin^{1

2}, Wei Yaojie^{1

2}, Jiang Yanan⁶, Jia Zhanrong⁶, Ma Chunyang^{1

2}, Gang Fangli^{1

2}, Xu Nan^{1

2}, Zhao Lingyun^{1

2}, Wang Xiumei^{1

2}, Wu Qiong^{3

4

5}, Lu Xiong⁶, Sun Xiaodan^{1

2}

Affiliations

¹ State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing, 100084, People's Republic of China.
² Key Laboratory of Advanced Materials of Ministry of Education of China, School of Materials Science and Engineering, Tsinghua University, Beijing, 100084, People's Republic of China.
³ MOE Key Laboratory of Bioinformatics, Tsinghua University, Beijing, 100084, People's Republic of China.
⁴ Center for Synthetic and Systems Biology, Tsinghua University, Beijing, 100084, People's Republic of China.
⁵ School of Life Sciences, Tsinghua University, Beijing, 100084, People's Republic of China.
⁶ Key Lab of Advanced Technologies of Materials of Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, People's Republic of China.

Abstract

The fate of mesenchymal stem cells (MSCs) is regulated by biological, physical and chemical signals. Developments in biotechnology and materials science promoted the occurrence of bioactive materials which can provide physical and chemical signals for MSCs to regulate their fate. In order to design and synthesize materials that can precisely regulate the fate of MSCs, the relationship between the properties of materials and the fate of mesenchymal stem cells need to be clarified, in which the detection of the fate of mesenchymal stem cells plays an important role. In the past 30 years, a series of detection technologies have been developed to detect the fate of MSCs regulated by bioactive materials, among which high-throughput technology has shown great advantages due to its ability to detect large amounts of data at one time. In this review, the latest research progresses of detecting the fate of MSCs regulated by bone bioactive materials (BBMs) are systematically reviewed from traditional technology to high-throughput technology which is emphasized especially. Moreover, current problems and the future development direction of detection technologies of the MSCs fate regulated by BBMs are prospected. The aim of this review is to provide a detection technical framework for researchers to establish the relationship between the properties of BMMs and the fate of MSCs, so as to help researchers to design and synthesize BBMs better which can precisely regulate the fate of MSCs.

Keywords: Bone bioactive materials; High-throughput technology; Proteomics; Single cell RNA-seq; Transcriptomics.

Publication types

Review