Expression of MTDH and IL-10 Is an Independent Predictor of Worse Prognosis in ER-Negative or PR-Negative Breast Cancer Patients

Pei-Yi Chu; Shin-Mae Wang; Po-Ming Chen; Feng-Yao Tang; En-Pei Isabel Chiang

doi:10.3390/jcm9103153

Expression of MTDH and IL-10 Is an Independent Predictor of Worse Prognosis in ER-Negative or PR-Negative Breast Cancer Patients

J Clin Med. 2020 Sep 29;9(10):3153. doi: 10.3390/jcm9103153.

Authors

Pei-Yi Chu^{1

2

3

4}, Shin-Mae Wang⁵, Po-Ming Chen^{6

7

8}, Feng-Yao Tang⁹, En-Pei Isabel Chiang^{7

8}

Affiliations

¹ School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei 242, Taiwan.
² Department of Pathology, Show Chwan Memorial Hospital, Changhua 500, Taiwan.
³ Department of Health Food, Chung Chou University of Science and Technology, Changhua 510, Taiwan.
⁴ National Institute of Cancer Research, National Health Research Institute, Tainan 704, Taiwan.
⁵ Department of General Surgery, Show Chwan Memorial Hospital, Changhua 500, Taiwan.
⁶ Research Assistant Center, Show Chwan Memorial Hospital, Changhua 500, Taiwan.
⁷ Department of Food Science and Biotechnology, National Chung Hsing University, Taichung 40402, Taiwan.
⁸ Innovation and Development Center of Sustainable Agriculture (IDCSA), National Chung Hsing University, Taichung 402, Taiwan.
⁹ Biomedical Science Laboratory, Department of Nutrition, China Medical University, Taichung 40402, Taiwan.

Abstract

(1) Background: Tumor hypoxia leads to metastasis and certain immune responses, and interferes with normal biological functions. It also affects glucose intake, down-regulates oxidative phosphorylation, and inhibits fatty-acid desaturation regulated by hypoxia-inducible factor 1α (HIF-1α). Although tumor hypoxia has been found to promote tumor metastasis, the roles of HIF-1α-regulated genes and their application are not completely integrated in clinical practice. (2) Methods: We examined the correlation between HIF-1α, metadherin (MTDH), and interleukin (IL)-10 mRNA expression, as well as their expression patterns in the prognosis of breast cancer using the Gene Expression Profiling Interactive Analysis (GEPIA) databases via a web interface; tissue microarrays (TMAs) were stained for MTDH and IL-10 protein expression using immunohistochemistry. (3) Results: HIF-1α, MTDH, and IL-10 mRNA expression are highly correlated and strongly associated with poor prognosis. MTDH and IL-10 protein expression of breast cancer patients usually harbored negative estrogen receptor (ER) or progesterone receptor (PR) status, and late-stage tumors have higher IL-10 expression. With regard to MTDH and IL-10 protein expression status for using univariate and multivariate analysis, the results showed that the protein expression of MTDH and IL-10 in ER-negative or PR-negative breast cancer patients have the worse prognosis. (4) Conclusions: we propose a new insight into hypoxia tumors in the metabolism and immune evidence for breast cancer therapy.

Keywords: IL-10; MTDH; breast cancer; hypoxia; survival.