Designing a Bioinert Surface by Simple Coating with Cholesterol End-Modified Poly(ethylene glycol)

Yuya Sone; Masayuki Nogawa; Shoichiro Asayama

doi:10.1021/acs.langmuir.0c02481

Designing a Bioinert Surface by Simple Coating with Cholesterol End-Modified Poly(ethylene glycol)

Langmuir. 2020 Oct 13;36(40):12045-12052. doi: 10.1021/acs.langmuir.0c02481. Epub 2020 Oct 1.

Authors

Yuya Sone¹, Masayuki Nogawa², Shoichiro Asayama¹

Affiliations

¹ Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan.
² Central Blood Institute, Blood Service Headquarters, Japanese Red Cross Society, 2-1-67 Tatsumi, Koto-ku, Tokyo 135-8521, Japan.

PMID: 33002363
DOI: 10.1021/acs.langmuir.0c02481

Abstract

A bioinert surface has been designed by simple coating with cholesterol end-modified poly(ethylene glycol), Chol-U-Pr-mPEG, using a cholesterol anchor. A poly(propylene) (PP) surface was immersed into the Chol-U-Pr-mPEG aqueous solution, where control mPEGs without cholesterol were not suitable for the design of bioinert surfaces. The resulting surfaces coated with Chol-U-Pr-mPEG above and below its critical micelle concentration were swollen and less swollen, respectively. Chol-U-Pr-mPEG with a molecular weight of 2000, Chol-U-Pr-mPEG (2k), formed a swollen layer with a thickness of 10-15 nm and adhered to the PP surface with an estimated K_d value of 4.4×10^-7 M. The resulting Chol-U-Pr-mPEG (2k)-coated surface with the swollen layer suppressed the adsorption of γ-globulin proteins and the adhesion of platelets in plasma. Although the PP surface coated with Chol-U-Pr-mPEG with a molecular weight of 5000, Chol-U-Pr-mPEG (5k), also suppressed the adsorption of γ-globulin proteins, the Chol-U-Pr-mPEG (5k)-coated PP surface did not suppress the adhesion of the platelets in plasma despite the existence of a swollen layer with a thickness of 20-25 nm. These results suggest that the Chol-U-Pr-mPEG-coated PP surface with an optimized swollen layer has been established as a bioinert surface by a facile method.