Protocol for Isolation of Cardiac Interstitial Cells from Adult Murine Hearts for Unbiased Single Cell Profiling

Elvira Forte; Sandra Daigle; Nadia A Rosenthal

doi:10.1016/j.xpro.2020.100077

Protocol for Isolation of Cardiac Interstitial Cells from Adult Murine Hearts for Unbiased Single Cell Profiling

STAR Protoc. 2020 Sep 18;1(2):100077. doi: 10.1016/j.xpro.2020.100077.

Authors

Elvira Forte¹, Sandra Daigle¹, Nadia A Rosenthal^{1

2}

Affiliations

¹ The Jackson Laboratory, Bar Harbor, 04609 Maine, USA.
² National Heart and Lung Institute, Imperial College London, SW72BX London, UK.

Abstract

Interstitial cells have a crucial role in cardiac fibrosis and repair of the mammalian heart. Single-cell profiling using droplet-based technology has revolutionized the investigation of cell states and identities. Here, we present a protocol for the efficient isolation of high-quality live nucleated non-cardiomyocytes from adult murine heart, for unbiased single-cell RNA sequencing using 10× Chromium technology. This protocol has been applied to homeostatic and injured hearts from different mouse strains. For complete details on the use and execution of this protocol, please refer to Forte et al. (2020).

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Culture Techniques / methods*
Cells, Cultured / cytology*
Mice
Myocardium / cytology*
Single-Cell Analysis / methods*

Abstract

Publication types

MeSH terms

Grants and funding