A Yorkshire swine (Sus scrofa domesticus) model for nerve regeneration and ischemia based on the sciatic nerve and femoral artery

Sarah E Kinsley; Stephen D Fernicola; Marvin E Dingle; Milfred S Williams; Jody M Richardson; Dennis Taylor; Jaira F de Vasconcellos; Timothy R Malone; Matthew R Blattner; Jonathan K Smith; Alli Oliver; Amory L Koch; Laura E Riddle; Cara Reiter; William E Culp; Edward J Caterson; Leon J Nesti; Simon G Talbot

doi:10.1016/j.aanat.2020.151587

A Yorkshire swine (Sus scrofa domesticus) model for nerve regeneration and ischemia based on the sciatic nerve and femoral artery

Ann Anat. 2021 Jan:233:151587. doi: 10.1016/j.aanat.2020.151587. Epub 2020 Sep 28.

Authors

Sarah E Kinsley¹, Stephen D Fernicola², Marvin E Dingle³, Milfred S Williams², Jody M Richardson², Dennis Taylor⁴, Jaira F de Vasconcellos⁴, Timothy R Malone³, Matthew R Blattner³, Jonathan K Smith³, Alli Oliver⁵, Amory L Koch⁵, Laura E Riddle⁵, Cara Reiter⁵, William E Culp⁵, Edward J Caterson¹, Leon J Nesti², Simon G Talbot⁶

Affiliations

¹ Division of Plastic Surgery, Brigham and Women's Hospital, Boston, MA, United States.
² Department of Surgery, Uniformed Services University of Health Sciences, Bethesda, MD, United States.
³ Walter Reed National Medical Center, Bethesda, MD, United States.
⁴ Department of Surgery, Uniformed Services University of Health Sciences, Bethesda, MD, United States; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United States.
⁵ Center for Laboratory Animal Medicine, Uniformed Services University of Health Sciences, Bethesda, MD, United States.
⁶ Division of Plastic Surgery, Brigham and Women's Hospital, Boston, MA, United States. Electronic address: sgtalbot@bwh.harvard.edu.

PMID: 32998029
DOI: 10.1016/j.aanat.2020.151587

Abstract

Animal studies are essential to biomedical research and the cornerstone is a reproducible animal model. While there are many reports on rodent peripheral nerve injury models, a large animal model is essential to confirm the effects of nerve regeneration over the longer distances of regeneration required in humans. Swine have long been used as a large animal model for other surgical and biomedical studies. This paper represents a novel neurovascular injury model in the Sus scrofa domesticus swine (American Yorkshire pig). This paper will describe our experience and recommendations with pre-operative, operative and post-operative protocols and our refinements to produce an effective model.

Keywords: American Yorkshire pig; Animal model; Nerve regeneration; Surgery.

MeSH terms

Animals
Disease Models, Animal
Femoral Artery* / surgery
Ischemia
Nerve Regeneration
Sciatic Nerve
Sus scrofa*
Swine