In Vivo Enrichment and Elimination of Circulating Tumor Cells by Using a Black Phosphorus and Antibody Functionalized Intravenous Catheter

Dou Wang; Chenchen Ge; Weiyuan Liang; Qinhe Yang; Quan Liu; Wei Ma; Lulin Shi; Hong Wu; Yuhua Zhang; Zongze Wu; Chaoying Wei; Luodan Huang; Zhiyuan Fang; Liping Liu; Shiyun Bao; Han Zhang

doi:10.1002/advs.202000940

In Vivo Enrichment and Elimination of Circulating Tumor Cells by Using a Black Phosphorus and Antibody Functionalized Intravenous Catheter

Adv Sci (Weinh). 2020 Jun 11;7(17):2000940. doi: 10.1002/advs.202000940. eCollection 2020 Sep.

Authors

Dou Wang^{1

2}, Chenchen Ge^{1

3}, Weiyuan Liang⁴, Qinhe Yang⁵, Quan Liu^{1

3}, Wei Ma¹, Lulin Shi¹, Hong Wu¹, Yuhua Zhang¹, Zongze Wu¹, Chaoying Wei¹, Luodan Huang¹, Zhiyuan Fang⁶, Liping Liu¹, Shiyun Bao¹, Han Zhang⁴

Affiliations

¹ Department of Hepatobiliary and Pancreatic Surgery The 2nd Clinical medical College (Shenzhen People's Hospital) of Jinan University Shenzhen 518020 China.
² Department of Biomedical Engineering Southern University of Science and Technology Shenzhen 518055 China.
³ Integrated Chinese and Western Medicine Postdoctoral research station Jinan University Guangzhou 510632 China.
⁴ Shenzhen Second People's Hospital The First Affiliated Hospital of Shenzhen University and Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province Shenzhen University Shenzhen 518060 China.
⁵ School of Traditional Chinese Medicine Jinan University Guangzhou 510632 China.
⁶ School of Biomedical and Pharmaceutical Sciences Guangdong University of Technology Guangzhou 510006 China.

Abstract

The circulating tumor cell (CTC) count is closely related to cancer recurrence and metastasis. The technology that can in vivo destroy CTCs may bring great benefits to patients, which is an urgent clinical demand. Here, a minimally invasive therapeutic intravenous catheter for in vivo enriching and photothermal killing of CTCs is developed. The surface of catheter is modified with anti-EpCAM antibody and the interior is filled with black phosphorus nanosheets (BPNSs). CTCs in the peripheral blood are captured by the catheter continually with the aid of circulation. The captured CTCs are used for downstream analyses or in vivo eliminated by the near-infrared (NIR) photothermal effect of BPNSs. A capture efficiency of 2.1% is obtained during the 5 min of treatment, and 100% of the captured CTCs are killed by following NIR light irradiation in both an in vitro closed-loop circulation system and an in vivo rabbit model. This cost-effective modality for lowering the CTCs burden can be a good supplement to traditional therapies, which holds great promise as an effective clinical intervention for cancer patients.

Keywords: EpCAM; black phosphorus; circulating tumor cells; intravenous catheters; photothermal therapy.