Lactobacillus plantarum KSFY06 and geniposide counteract montmorillonite-induced constipation in Kunming mice

Yi Gan; Jie Liang; Wenjing Diao; Xianrong Zhou; Jianfei Mu; Liang Pang; Fang Tan; Xin Zhao

doi:10.1002/fsn3.1814

Lactobacillus plantarum KSFY06 and geniposide counteract montmorillonite-induced constipation in Kunming mice

Food Sci Nutr. 2020 Aug 7;8(9):5128-5137. doi: 10.1002/fsn3.1814. eCollection 2020 Sep.

Authors

Yi Gan^{1

2

3}, Jie Liang⁴, Wenjing Diao^{1

2

3}, Xianrong Zhou^{1

2

3}, Jianfei Mu^{1

2

3}, Liang Pang⁵, Fang Tan⁶, Xin Zhao^{1

2

3}

Affiliations

¹ Chongqing Collaborative Innovation Center for Functional Food Chongqing University of Education Chongqing China.
² Chongqing Engineering Research Center of Functional Food Chongqing University of Education Chongqing China.
³ Chongqing Engineering Laboratory for Research and Development of Functional Food Chongqing University of Education Chongqing China.
⁴ Department of Pediatrics Chongqing Traditional Chinese Medicine Hospital Chongqing China.
⁵ Department of Oral and Maxillofacial Surgery The Affiliated Hospital of Stomatology Chongqing Medical University Chongqing China.
⁶ Department of Public Health Our Lady of Fatima University Valenzuela Philippines.

Abstract

Constipation is a common clinical manifestation of digestive system disorders and occurs worldwide. This study investigated the ability of Lactobacillus plantarum KSFY06 (LP-KSFY06) to promote the action of geniposide in preventing montmorillonite-induced constipation in Kunming mice, with the aim of providing a successful solution. The effects of LP-KSFY06 and geniposide on constipation were measured, and the results showed that the protective effect of geniposide on constipation was enhanced by LP-KSFY06 and that the combination resulted in increased weight, moisture content, and particle number of feces. The first black stool defecation time was decreased from 182 min to 87 min, which clearly indicates that defecating difficulty was alleviated in constipated mice. The synergic intervention of LP-KSFY06 and geniposide (LP + G) assisted in maintaining the body weight of constipated mice. The LP + G intervention significantly increased serum levels of motilin (MTL, 167.8 pg/ml), acetylcholinesterase (AChE, 45.3 pg/ml), substance P (SP, 61.0 pg/ml), vasoactive intestinal peptide (VIP, 70.5 pg/ml), endothelin-1 (ET-1, 16.1 pg/ml), and gastrin (73.0 pg/ml) and remarkably decreased somatostatin (SS, 35.2 pg/ml) when compared to those indexes in the LP-KSFY06 group and geniposide group. The LP + G treatment also significantly increased the mRNA expression of cluster of differentiation 117 (c-Kit), stem cell factor (SCF), glial cell-derived neurotrophic factor (GDNF), and remarkably downregulated the expression of inducible nitric oxide synthase (iNOS), transient receptor potential vanilloid-1 (TRPV1), and cyclooxygenase-2 (COX-2). The experimental results showed that the combination treatment has the strongest prevention effect against constipation, and LP-KSFY06 promotes the ability of geniposide to prevent constipation. Therefore, LP-KSFY06 is a potential probiotic strain with the capacity to prevent montmorillonite-induced constipation.

Keywords: Lactobacillus plantarum; constipation; geniposide; mRNA; montmorillonite.