Biomimetic synthesis of calcium carbonate under phenylalanine: Control of polymorph and morphology

Abstract

In biomineralization, organisms have the abilities to produce biominerals with superior properties. One of the most attractive features of biominerals is the presence of the proteins consisting of different contents of amino acids in crystals. In the present work, L-phenylalanine (Phe) was used as an additive for the controllable crystallization of calcium carbonate (CaCO₃). The obtained CaCO₃ crystals were characterized by field emission scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), elemental analysis and high-resolution transmission electron microscopy (HRTEM). The experimental results suggest that single calcite crystals are formed at low Phe concentrations. High concentrations of Phe inhibit the nucleation and growth of calcite, and promote the formation of vaterite crystals with solid or hollow structures. The morphology and crystal form of CaCO₃ are also significantly affected by the flow rate of CO₂. After that, a possible mechanism (competition mechanism) action of Phe in the formation of CaCO₃ is proposed. Finally, the effects of temperature on the formation of vaterite were determined to explore the growth mechanism of hexagonal vaterite. The work of controlling the preparation of CaCO₃ crystals in the presence of Phe will help us to imitate and learn nature, and bring new insights into understanding bionics. Meanwhile, it provides a new method for the synthesis of CaCO₃ biomaterials with different crystal forms and morphologies.

Keywords: Calcite; Calcium carbonate; Morphology; Phenylalanine; Polymorph; Vaterite.