Integrative Transcriptome and Proteome Analysis Identifies Major Molecular Regulation Pathways Involved in Ramie (Boehmeria nivea (L.) Gaudich) under Nitrogen and Water Co-Limitation

Jikang Chen; Gang Gao; Ping Chen; Kunmei Chen; Xiaofei Wang; Lianyang Bai; Chunming Yu; Aiguo Zhu

doi:10.3390/plants9101267

Integrative Transcriptome and Proteome Analysis Identifies Major Molecular Regulation Pathways Involved in Ramie (Boehmeria nivea (L.) Gaudich) under Nitrogen and Water Co-Limitation

Plants (Basel). 2020 Sep 25;9(10):1267. doi: 10.3390/plants9101267.

Authors

Jikang Chen^{1

2

3}, Gang Gao^{2

3}, Ping Chen^{2

3}, Kunmei Chen^{2

3}, Xiaofei Wang^{2

3}, Lianyang Bai^{1

4}, Chunming Yu^{2

3}, Aiguo Zhu^{2

3}

Affiliations

¹ Longping Branch, Graduate School of Hunan University, Changsha 410082, China.
² Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China.
³ National Breeding Center for Bast Fiber Crops, Changsha 410125, China.
⁴ Hunan Academy of Agricultural Sciences, Changsha 410125, China.

Abstract

Water and N are the most important factors affecting ramie (Boehmeria nivea (L.) Gaudich) growth. In this study, de novo transcriptome assembly and Tandem Mass Tags (TMT) based quantitative proteome analysis of ramie under nitrogen and water co-limitation conditions were performed, and exposed to treatments, including drought and N-deficit (WdNd), proper water but N-deficit (WNd), proper N but drought (WdN), and proper N and water (CK), respectively. A total of 64,848 unigenes (41.92% of total unigenes) were annotated in at least one database, including NCBI non-redundant protein sequences (Nr), Swiss-Prot, Protein family (Pfam), Gene Ontology (GO) and KEGG Orthology (KO), and 4268 protein groups were identified. Most significant changes in transcript levels happened under water-limited conditions, but most significant changes in protein level happened under water-limited conditions only with proper N. Poor correlation between differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) was observed in ramie responding to the treatments. DEG/DEP regulation patterns related to major metabolic processes responding to water and N deficiency were analyzed, including photosynthesis, ethylene responding, glycolysis, and nitrogen metabolism. Moreover, 41 DEGs and 61 DEPs involved in regulating adaptation of ramie under water and N stresses were provided in the study, including DEGs/DEPs related to UDP-glucuronosyhransferase (UGT), ATP synthase, and carbonate dehydratase. The strong dependency of N-response of ramie on water conditions at the gene and protein levels was highlighted. Advices for simultaneously improving water and N efficiency in ramie were also provided, especially in breeding N efficient varieties with drought resistance. This study provided extensive new information on the transcriptome, proteome, their correlation, and diversification in ramie responding to water and N co-limitation.

Keywords: nitrogen and water co-limitation; proteome; ramie (Boehmeria nivea (L.) Gaudich); synergistic effects; transcriptome.

Abstract

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