Prevalence of Vibrio parahaemolyticus Causing Acute Hepatopancreatic Necrosis Disease of Shrimp in Shrimp, Molluscan Shellfish and Water Samples in the Mekong Delta, Vietnam

Tran Thi Hong To; Haruka Yanagawa; Nguyen Khanh Thuan; Du Minh Hiep; Doan Van Cuong; Ly Thi Lien Khai; Takahide Taniguchi; Ryoichi Kubo; Hideki Hayashidani

doi:10.3390/biology9100312

Prevalence of Vibrio parahaemolyticus Causing Acute Hepatopancreatic Necrosis Disease of Shrimp in Shrimp, Molluscan Shellfish and Water Samples in the Mekong Delta, Vietnam

Biology (Basel). 2020 Sep 25;9(10):312. doi: 10.3390/biology9100312.

Authors

Affiliations

¹ Division of Animal Life Science, Institute of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
² School of Agriculture and Aquaculture, Tra Vinh University, 126, Hamlet 4, Ward 5, Tra Vinh, Vietnam.
³ Faculty of Agriculture, Can Tho University, Campus II, 3/2 Street, Ninh Kieu District, Can Tho City, Vietnam.
⁴ Research Institute for Aquaculture No.2, 116, Ward Da kao, District 1, Ho Chi Minh, Vietnam.
⁵ Kanto Chemical Co. Inc., 2-1, Nihonbashi Muromachi 2-chome, Chuo-ku, Tokyo 103-0022, Japan.

Abstract

A total of 481 samples, including 417 shrimp and molluscan shellfish samples from retail shops and farms and 64 water samples from shrimp and molluscan shellfish farms in the Mekong Delta located the southern part of Vietnam, were examined for the presence of Vibrio parahaemolyticus (Vp_AHPND) caused acute haepatopancreatic necrosic disease (AHPND) in shrimp. Vp_AHPND strains were isolated in two of 298 (0.7%) molluscan shellfish samples from retail shops, seven of 71 (9.9%) shrimp samples from shrimp ponds, and two of 42 (4.8%) water samples from shrimp ponds. Vp_AHPND strains were classified into two types of O antigen, including O1 and O3, in which O1 was the predominant. Vp_AHPND strains isolated showed high resistance rates to colistin (100%), ampicillin (93.8%), and streptomycin (87.5%). These results indicate that Vp_AHPND is widely prevalent in environment in the Mekong Delta, Vietnam.

Keywords: Vibrio parahaemolyticus; antimicrobial resistance; pirvp genes; serotype.