Plant leaves can intercept and directly absorb nanoparticles (NPs) that deposit on their surface, which can lead severe phytotoxicity. However, there is a large blind spot when it comes to the fate and phytotoxicity of NPs after leaf exposure, even though foliar uptake is likely to occur. In this study, lettuce leaves (Lactuca sativa L. var. ramosa Hort.) were exposed to different concentrations of copper-oxide NPs (CuO-NPs, 0, 100, and 1000 mg L-1) for 5, 10, and 15 days. Foliar uptake, subcellular distribution, chemical forms, and impact of CuO-NPs on nutrient status, antioxidant systems, and lettuce growth were examined. Substantially elevated Cu levels were observed in lettuce leaves (up to 6350 mg kg-1), which was one magnitude greater than that in the roots (up to 525 mg kg-1). Cu translocation factors from leaves to roots ranged from 1.80 to 15.6%. The application of CuO-NPs severely inhibited lettuce growth and altered the nutrient status in plants (especially Mn, K, and Ca). Moreover, CuO-NPs increased H2O2 generation, malonaldehyde level (on the 5th and 10th day of exposure), and catalase activity (on the 15th day of exposure) in lettuce leaves. The Cu concentrations in subcellular fractions were ranked: cell wall ≈ organelles > soluble fraction in lettuce leaves, and organelles > cell wall > soluble fraction in lettuce roots. Undissolved Cu forms were predominant in lettuce, which may have helped to reduce the Cu's mobility and phytotoxicity in the plant. The findings of this study will be of great interest in areas with high levels of metal-NPs in the atmosphere.
Keywords: Chemical speciation; Foliar exposure; Nanoparticles; Oxidative stress; Subcellular distribution.