Enhancement of the biohydrogen production performance from mixed substrate by photo-fermentation: Effects of initial pH and inoculation volume ratio

Xueting Zhang; Danping Jiang; Huan Zhang; Yanjin Wang; Zhiping Zhang; Chaoyang Lu; Quanguo Zhang

doi:10.1016/j.biortech.2020.124153

Enhancement of the biohydrogen production performance from mixed substrate by photo-fermentation: Effects of initial pH and inoculation volume ratio

Bioresour Technol. 2021 Jan:319:124153. doi: 10.1016/j.biortech.2020.124153. Epub 2020 Sep 25.

Authors

Xueting Zhang¹, Danping Jiang², Huan Zhang¹, Yanjin Wang³, Zhiping Zhang¹, Chaoyang Lu¹, Quanguo Zhang⁴

Affiliations

¹ Key Laboratory of New Materials and Facilities for Rural Renewable Energy, MOA of China, Henan Agricultural University, Zhengzhou 450002, China.
² Key Laboratory of New Materials and Facilities for Rural Renewable Energy, MOA of China, Henan Agricultural University, Zhengzhou 450002, China; Institute of Agricultural Engineering, Huanghe S & T University, Zhengzhou 450006, China.
³ Institute of Agricultural Engineering, Huanghe S & T University, Zhengzhou 450006, China.
⁴ Key Laboratory of New Materials and Facilities for Rural Renewable Energy, MOA of China, Henan Agricultural University, Zhengzhou 450002, China. Electronic address: zquanguo@163.com.

PMID: 32987277
DOI: 10.1016/j.biortech.2020.124153

Abstract

Co-digestion of substrates can improve hydrogen yield (HY) by adjusting carbon nitrogen ratio (C/N) of fermentation substrates. This study evaluated the enhancement of hydrogen production from co-digestion of duckweed and corn straw via photo-fermentation. The maximum HY of 78.0 mL/g Total solid (TS) was obtained from the mixed ratio of 5:1 (C/N of 13.2), which was 25.4% and 29.6% higher than those of single substrate of duckweed and corn straw, respectively. The effects of initial pH and inoculation volume ratio (IVR) on co-digestion photo-fermentative hydrogen production (CD-PFHP) from duckweed and corn straw were further studied. A maximum HY of 85.6 mL/g TS was achieved under the optimal condition (initial pH 8, IVR 20%, mix ratio of duckweed and corn straw of 5:1). Additionally, both mix ratio and initial pH showed statistical difference (p < 0.05). Acetic acid and butyric acid were found to be the main metabolic by-products during CD-PFHP.

Keywords: C/N; Co-digestion; Initial pH; Inoculation volume ratio; Photo-fermentative.

MeSH terms

Fermentation
Hydrogen*
Hydrogen-Ion Concentration
Nitrogen
Zea mays*

Substances

Hydrogen
Nitrogen