A carbon dot-based fluorometric probe for oxytetracycline detection utilizing a Förster resonance energy transfer mechanism

Yanzhao Fu; Li Huang; Shaojing Zhao; Xuejian Xing; Minhuan Lan; Xiangzhi Song

doi:10.1016/j.saa.2020.118947

A carbon dot-based fluorometric probe for oxytetracycline detection utilizing a Förster resonance energy transfer mechanism

Spectrochim Acta A Mol Biomol Spectrosc. 2021 Feb 5:246:118947. doi: 10.1016/j.saa.2020.118947. Epub 2020 Sep 17.

Authors

Yanzhao Fu¹, Li Huang¹, Shaojing Zhao², Xuejian Xing², Minhuan Lan³, Xiangzhi Song⁴

Affiliations

¹ Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China; Shenzhen Research Institute of Central South University, Shenzhen 518057, PR China.
² Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
³ Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China; Shenzhen Research Institute of Central South University, Shenzhen 518057, PR China. Electronic address: minhuanlan@csu.edu.cn.
⁴ Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China. Electronic address: xzsong@csu.edu.cn.

PMID: 32987267
DOI: 10.1016/j.saa.2020.118947

Abstract

A carbon dot (CD)-based fluorometric probe for oxytetracycline (OTC) detection utilizing a Förster resonance energy transfer (FRET) mechanism was firstly developed. Upon addition of OTC, the blue fluorescence of the CDs peaked at 405 nm was quickly quenched, with a new fluorescence peaked at 505 nm attributed to OTC was observed within 30 s. A visual fluorescent color change of the CDs solution from blue to green was discerned under a 365 nm UV light irradiation. The CDs displayed a high sensitivity and selectivity toward OTC with a low detection limit of 0.41 μM. Furthermore, the probe was applied to detect OTC in water, milk, and pork samples with a satisfied recovery.

Keywords: Antibiotics; Carbon dots; FRET; Fluorometric probe.

MeSH terms

Carbon
Fluorescence Resonance Energy Transfer
Fluorometry
Oxytetracycline*
Quantum Dots*

Substances

Carbon
Oxytetracycline