An integrated exploration of pharmacological potencies of Bischofia javanica (Blume) leaves through experimental and computational modeling

Md Riad Chowdhury; Kamrul Hasan Chowdhury; Nujhat Binte Hanif; Mohammed Abu Sayeed; Jannatul Mouah; Iftekher Mahmud; A T M Mostafa Kamal; Md Nazim Uddin Chy; Md Adnan

doi:10.1016/j.heliyon.2020.e04895

An integrated exploration of pharmacological potencies of Bischofia javanica (Blume) leaves through experimental and computational modeling

Heliyon. 2020 Sep 14;6(9):e04895. doi: 10.1016/j.heliyon.2020.e04895. eCollection 2020 Sep.

Authors

Md Riad Chowdhury¹, Kamrul Hasan Chowdhury¹, Nujhat Binte Hanif¹, Mohammed Abu Sayeed¹, Jannatul Mouah¹, Iftekher Mahmud², A T M Mostafa Kamal¹, Md Nazim Uddin Chy¹, Md Adnan^{1

3}

Affiliations

¹ Department of Pharmacy, International Islamic University Chittagong, Chittagong, 4318, Bangladesh.
² Department of Chemistry, Wayne State University, Detroit, Michigan, 48202, United States.
³ Department of Bio-Health Technology, College of Biomedical Science, Kangwon National University, Chuncheon, 24341, Republic of Korea.

Abstract

Bischofia javanica (Blume), an edible wild plant, has both prospective nutraceutical and therapeutic properties. Here, we intended to explore the pharmacological potentials of the methanol extract of B. javanica (MEBJ) through integrated approaches. Phytochemical screening revealed the presence of important phytoconstituents which were found to be safe during cytotoxicity analysis. The sedative potential of MEBJ (200 and 400 mg/kg) was determined by employing open field, hole cross, and thiopental sodium-induced sleeping time tests, where a significant reduction of the locomotor performance and an enhancement in the duration of sleeping have been observed, respectively. In addition, mice treated with MEBJ exhibited superior exploration during both elevated plus maze and hole board tests. In parallel, anti-diabetic potency was investigated via alpha-amylase inhibitory assay, where a dose-response increase in the percentage of inhibition has been marked. A similar response, such as an increased percentage of clot lysis, was observed during the thrombolytic test. Furthermore, molecular docking was performed with the identified compounds, demonstrated strong binding affinities to the target receptors of the experiments as mentioned above. Also, ADME/T and toxicological parameters verified the drug-like properties of the identified compounds. Collectively, these results indicate bioactivity of Bischofia javanica, which can be a potential candidate in the food and pharmaceutical industries.

Keywords: Anti-diabetic; Bioinformatics; Bischofia javanica; Cytotoxic; Evidence-based medicine; Molecular docking; Neuroscience; Pharmaceutical science; Pharmacology; Sedative and anxiolytic; Thrombolytic; Toxicology.