IGF2BP3 May Contributes to Lung Tumorigenesis by Regulating the Alternative Splicing of PKM

Huang Xueqing; Zhang Jun; Jiang Yueqiang; Liao Xin; Hu Liya; Fang Yuanyuan; Zhang Yuting; Zeng Hao; Wu Hua; Liu Jian; Yin Tiejun

doi:10.3389/fbioe.2020.00679

IGF2BP3 May Contributes to Lung Tumorigenesis by Regulating the Alternative Splicing of PKM

Front Bioeng Biotechnol. 2020 Sep 2:8:679. doi: 10.3389/fbioe.2020.00679. eCollection 2020.

Authors

Huang Xueqing¹, Zhang Jun¹, Jiang Yueqiang¹, Liao Xin¹, Hu Liya¹, Fang Yuanyuan¹, Zhang Yuting¹, Zeng Hao¹, Wu Hua¹, Liu Jian¹, Yin Tiejun¹

Affiliation

¹ Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Abstract

RNA binding proteins (RBPs) play a key role in genome regulation. Here we report the post-transcript regulation of IGF2BP3, which belongs to the insulin-like growth factor 2 mRNA binding protein family. We used iRIP-seq and RNA-seq to analyze the transcript regulation and alternative splicing on IGF2BP3 treated with overexpression cells and control. Overexpressed IGF2BP3 has broadly increased genes expression which involved in G-protein coupled receptor signaling pathway, positive regulation of cell proliferation, and signal transduction. IGF2BP3 regulated alternative splicing of multiple genes mainly clustered at response to hypoxia, negative regulation of transcription, and embryonic development. This study first provides alternative splicing analysis on transcription level of IGF2BP3 regulation, which laid the foundation for later research on IGF2BP3 critical functions.

Keywords: IGF2BP3; IGF2BP3/IMP3; alternative splicing; cancer therapy 3; iRIP-seq; lung tumorigenesis.