Nup84 persists within the nuclear envelope of the rice blast fungus, Magnaporthe oryzae, during mitosis

Mariel A Pfeifer; Chang Hyun Khang

doi:10.1016/j.fgb.2020.103472

Nup84 persists within the nuclear envelope of the rice blast fungus, Magnaporthe oryzae, during mitosis

Fungal Genet Biol. 2021 Jan:146:103472. doi: 10.1016/j.fgb.2020.103472. Epub 2020 Sep 25.

Authors

Mariel A Pfeifer¹, Chang Hyun Khang²

Affiliations

¹ Department of Plant Biology, 2502 Miller Plant Sciences, University of Georgia, Athens, GA 30602-7271, USA.
² Department of Plant Biology, 2502 Miller Plant Sciences, University of Georgia, Athens, GA 30602-7271, USA. Electronic address: ckhang@uga.edu.

PMID: 32980454
DOI: 10.1016/j.fgb.2020.103472

Abstract

The arrangement of the nuclear envelope in the rice blast fungus, Magnaporthe oryzae, was previously undetermined. Here, we identified two conserved components of the nuclear envelope, a core nucleoporin, Nup84, and an inner nuclear membrane protein, Src1. Live-cell super-resolution structured illumination microscopy revealed that Nup84-tdTomato and Src1-EGFP colocalized within the nuclear envelope during interphase and that Nup84-tdTomato remained associated with the dividing nucleus. We also found that appressorium development involved a mitotic nuclear migration event through the germ tube.

Keywords: Appressorium; Intermediate mitosis; Nucleoporin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ascomycota / genetics*
Ascomycota / pathogenicity
Biological Transport / genetics
Fungal Proteins / genetics*
Hyphae / genetics
Hyphae / pathogenicity
Mitosis / genetics*
Nuclear Envelope / genetics
Nuclear Pore Complex Proteins / genetics*
Oryza / genetics
Oryza / microbiology
Plant Diseases / genetics
Plant Diseases / microbiology

Substances

Fungal Proteins
Nuclear Pore Complex Proteins

Supplementary concepts

Pyricularia oryzae