Structure of the O-antigen of a halophilic bacterium Salinicola salarius HO-14

Andrei V Perepelov; Ekaterina M Semenova; Alexander S Shashkov; Andrei V Filatov; Tamara N Nazina

doi:10.1016/j.carres.2020.108149

Structure of the O-antigen of a halophilic bacterium Salinicola salarius HO-14

Carbohydr Res. 2020 Nov:497:108149. doi: 10.1016/j.carres.2020.108149. Epub 2020 Sep 6.

Authors

Andrei V Perepelov¹, Ekaterina M Semenova², Alexander S Shashkov³, Andrei V Filatov³, Tamara N Nazina²

Affiliations

¹ N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation. Electronic address: andreivperepelov@gmail.com.
² Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, 117312, Russian Federation.
³ N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.

PMID: 32979627
DOI: 10.1016/j.carres.2020.108149

Abstract

The structure of the O-polysaccharide of an aerobic halophilic bacterium Salinicola salarius HO-14 isolated from a heavy oil reservoir with highly mineralized water was determined. The neutral O-polysaccharide of strain HO-14 was isolated from the lipopolysaccharide and studied by sugar analysis and NMR spectroscopy. The linear tetrasaccharide repeating unit was found to have the following structure: →2)-α-l-Rhap-(1 → 3)-β-l-Rhap-(1 → 2)-α-l-Rhap-(1 → 2)-α-d-Manp-(1→.

Keywords: Bacterial polysaccharide structure; Halophile; O-antigen structure; Salinicola salarius.

MeSH terms

Carbohydrate Conformation
Carbohydrate Sequence
Halomonadaceae / chemistry*
Models, Molecular
O Antigens / chemistry*
Water / chemistry

Substances

O Antigens
Water

Supplementary concepts

Salinicola salarius