Determination of SARS-CoV-2 antibodies with assays from Diasorin, Roche and IDvet

J Virol Methods. 2021 Jan:287:113978. doi: 10.1016/j.jviromet.2020.113978. Epub 2020 Sep 23.

Abstract

There is an ongoing need for highly reliable serological assays to detect individuals with past SARS-CoV-2 infection. Using 75 sera from patients tested positive or negative by SARS-CoV-2 PCR, we investigated the sensitivity and specificity of the Liaison SARS-CoV-2 S1/S2 IgG assay (DiaSorin), the Elecsys Anti-SARS-CoV-2 assay (Roche), and the ID Screen SARS-CoV-2-N IgG indirect kit (IDVet). We determined a sensitivity of 95.5 %, 95.5 %, and 100 % and a specificity of 90.5 %, 96.2 %, and 92.5 % for the DiaSorin assay, the Roche assay, and the IDVet assay, respectively. We conclude that serologic assays combining very high sensitivity and specificity are still not commercially available for SARS-CoV-2. For maximizing sensitivity and specificity of SARS-CoV-2 serological diagnostics, the combination of two assays may be helpful.

Keywords: COVID-19; ELISA; SARS-CoV-2; Serology.

Publication types

  • Comparative Study

MeSH terms

  • Antibodies, Viral / blood
  • COVID-19 / blood*
  • COVID-19 Serological Testing / methods*
  • Humans
  • Immunoassay
  • Immunoglobulin G / blood
  • SARS-CoV-2 / immunology*
  • Sensitivity and Specificity

Substances

  • Antibodies, Viral
  • Immunoglobulin G