Knockdown of circ_HIPK3 inhibits tumorigenesis of hepatocellular carcinoma via the miR-582-3p/DLX2 axis

Biochem Biophys Res Commun. 2020 Dec 10;533(3):501-509. doi: 10.1016/j.bbrc.2020.09.050. Epub 2020 Sep 22.

Abstract

Hepatocellular carcinoma (HCC) is the most common type in the sub-classification of liver cancer. Circular RNAs (circRNAs) play a fundamental role in tumor occurrence and progression. This research aimed to investigate the role and molecular basis of circRNA homeodomain-interacting protein kinase 3 (circ_HIPK3) in HCC. Circ_HIPK3 and DLX2 levels were enhanced, and miR-582-3p level was reduced in HCC tissues and cells. Silencing of circ_HIPK3 impeded proliferation, migration and invasion and expedited apoptosis in HCC cells. Furthermore, circ_HIPK3 modulated HCC progression via sponging miR-582-3p, and miR-582-3p suppressed HCC progression via targeting DLX2. Moreover, circ_HIPK3 knockdown inhibited tumor growth in vivo. Circ_HIPK3 facilitated HCC progression by mediating miR-582-3p/DLX2 pathway, suggesting a new potential biomarker for HCC treatment.

Keywords: DLX2; Hepatocellular carcinoma; circ_HIPK3; miR-582-3p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis
  • Carcinoma, Hepatocellular / genetics*
  • Carcinoma, Hepatocellular / pathology
  • Carcinoma, Hepatocellular / secondary
  • Cell Line, Tumor
  • Cell Proliferation
  • Cells, Cultured
  • Gene Expression Regulation, Neoplastic*
  • Gene Knockdown Techniques
  • Homeodomain Proteins / genetics*
  • Homeodomain Proteins / metabolism
  • Humans
  • Liver Neoplasms / genetics*
  • Liver Neoplasms / metabolism
  • Liver Neoplasms / pathology
  • Mice, Nude
  • MicroRNAs / metabolism*
  • RNA, Circular / metabolism
  • RNA, Circular / physiology*
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism

Substances

  • DLX2 protein, human
  • Homeodomain Proteins
  • MIRN582 microRNA, human
  • MicroRNAs
  • RNA, Circular
  • Transcription Factors