Bioavailability and phytotoxicity of rare earth metals to Triticum aestivum under various exposure scenarios

Abstract

It is a daunting challenge to predict toxicity and accumulation of rare earth metals (REMs) in different exposure scenarios (e.g., varying water chemistry and metal combinations). Herein, we investigated the toxicity and uptake of La and Ce in the presence of various levels of Ca, Mg, Na, K, and at different pH values, as well as the combined effects of La and Ce in wheat Triticum aestivum. Major cations (Ca²⁺ and Mg²⁺) significantly mitigated the toxicity and accumulation of La³⁺/Ce³⁺. Toxicity and uptake of La, Ce, and La-Ce mixtures could be well quantified by the multi-metal biotic ligand model (BLM) and by the Langmuir-type uptake model with the consideration of the competitive effects of Ca²⁺ and Mg²⁺, with more than 85.1% of variations explained. The derived binding constants of Ca, Mg, La, and Ce to wheat root were respectively 3.87, 3.59, 6.97, and 6.48 on the basis of toxicity data, and 3.23, 2.84, 6.07, and 5.27 on the basis of uptake data. The use of the alternative WHAM-F_tox approach, requiring fewer model parameters than the BLM but with similar Akaike information criterion (AIC) values, successfully predicted the toxicity and accumulation of La/Ce as well as toxicity of La-Ce mixtures, with at least 76.4% of variations explained. However, caution should be taken when using this approach to explain the uptake of La-Ce mixtures. Our results provided promising tools for delineating REMs toxicity/uptake in the presence of other toxicity-modifying factors or in mixture scenarios.

Keywords: BLM; Bioavailability; Rare earth metals; Toxicity; Uptake; WHAM-F(tox).