Multilocus Sequence Typing (MLST) and Whole Genome Sequencing (WGS) of Listeria monocytogenes and Listeria innocua

Methods Mol Biol. 2021:2220:89-103. doi: 10.1007/978-1-0716-0982-8_7.

Abstract

Nucleotide sequence-based methods focusing on the single-nucleotide polymorphisms (SNPs) of Listeria monocytogenes and L. innocua housekeeping genes (multilocus sequence typing) and in the core genome (core genome MLST) facilitate the rapid and interlaboratory comparison in open accessible databases as provided by Institute Pasteur ( https://bigsdb.web.pasteur.fr/listeria/listeria.html ). Strains can be compared on a global level and help to track forward and trace backward pathogen contamination events in food processing facilities and in outbreak scenarios.

Keywords: Housekeeping genes; Listeria monocytogenes; Multilocus sequence typing; Single-nucleotide polymorphisms; Whole genome sequencing.

MeSH terms

  • DNA, Bacterial / genetics
  • Food Microbiology
  • Genome, Bacterial
  • Humans
  • Listeria / genetics*
  • Listeria monocytogenes / genetics*
  • Listeriosis / microbiology
  • Multilocus Sequence Typing / methods*
  • Polymerase Chain Reaction / methods
  • Polymorphism, Single Nucleotide
  • Whole Genome Sequencing / methods*

Substances

  • DNA, Bacterial

Supplementary concepts

  • Listeria innocua