Whole-Genome Sequence of SARS-CoV-2 Isolate Siena-1/2020

Maria Grazia Cusi; David Pinzauti; Claudia Gandolfo; Gabriele Anichini; Gianni Pozzi; Francesco Santoro

doi:10.1128/MRA.00944-20

Whole-Genome Sequence of SARS-CoV-2 Isolate Siena-1/2020

Microbiol Resour Announc. 2020 Sep 24;9(39):e00944-20. doi: 10.1128/MRA.00944-20.

Authors

Maria Grazia Cusi^{1

2}, David Pinzauti¹, Claudia Gandolfo¹, Gabriele Anichini¹, Gianni Pozzi¹, Francesco Santoro^{3

2}

Affiliations

¹ Department of Medical Biotechnologies, University of Siena, Siena, Italy.
² UOC Microbiologia e Virologia, Azienda Ospedaliera Universitaria Senese, Siena, Italy.
³ Department of Medical Biotechnologies, University of Siena, Siena, Italy santorof@unisi.it.

Abstract

The complete genome sequence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) isolate Siena-1/2020 was obtained by Nanopore sequencing, combining the direct RNA sequencing and amplicon sequencing approaches. The isolate belongs to the B1.1 lineage, which is prevalent in Europe, and contains a mutation in the spike protein coding sequence leading to the D614G amino acid change.