Daptomycin resistant Enterococcus faecalis has a mutation in liaX, which encodes a surface protein that inhibits the LiaFSR systems and cell membrane remodeling

Yusuke Ota; Kazuki Furuhashi; Wataru Hayashi; Nachi Hirai; Jinko Ishikawa; Osanori Nagura; Katsumasa Yamanaka; Kazuto Katahashi; Kotaro Aoki; Noriyuki Nagano; Masato Maekawa

doi:10.1016/j.jiac.2020.09.004

Daptomycin resistant Enterococcus faecalis has a mutation in liaX, which encodes a surface protein that inhibits the LiaFSR systems and cell membrane remodeling

J Infect Chemother. 2021 Jan;27(1):90-93. doi: 10.1016/j.jiac.2020.09.004. Epub 2020 Sep 22.

Authors

Affiliations

¹ Department of Laboratory Medicine, Hamamatsu University School of Medicine, Shizuoka, Japan. Electronic address: yota7sh@hama-med.ac.jp.
² Department of Laboratory Medicine, Hamamatsu University School of Medicine, Shizuoka, Japan. Electronic address: k.furu@hama-med.ac.jp.
³ Department of Medical Sciences, Shinshu University Graduate School of Medicine, Science and Technology, Nagano, Japan. Electronic address: 19hm205j@shinshu-u.ac.jp.
⁴ Department of Laboratory Medicine, Hamamatsu University School of Medicine, Shizuoka, Japan. Electronic address: nachirai@hama-med.ac.jp.
⁵ Department of Laboratory Medicine, Hamamatsu University School of Medicine, Shizuoka, Japan. Electronic address: jin0406@hama-med.ac.jp.
⁶ Department of Laboratory Medicine, Hamamatsu University School of Medicine, Shizuoka, Japan. Electronic address: rikyo@hama-med.ac.jp.
⁷ Department of Laboratory Medicine, Hamamatsu University School of Medicine, Shizuoka, Japan. Electronic address: k-yama@hama-med.ac.jp.
⁸ Second Department of Surgery, Hamamatsu University School of Medicine, Hamamatsu University School of Medicine, Shizuoka, Japan. Electronic address: kazukata@hama-med.ac.jp.
⁹ Department of Microbiology and Infectious Diseases, Toho University School of Medicine, Tokyo, Japan. Electronic address: kotaro.aoki@med.toho-u.ac.jp.
¹⁰ Department of Medical Sciences, Shinshu University Graduate School of Medicine, Science and Technology, Nagano, Japan. Electronic address: naganon@shinshu-u.ac.jp.
¹¹ Department of Laboratory Medicine, Hamamatsu University School of Medicine, Shizuoka, Japan. Electronic address: mmaekawa@hama-med.ac.jp.

PMID: 32972829
DOI: 10.1016/j.jiac.2020.09.004

Abstract

The emergence of daptomycin (DAP) resistant Enterococcus species has increased worldwide, but the mechanisms for DAP resistance are not fully understood. We report a case of DAP resistant Enterococcus faecalis, from a clinical sample of a patient with diabetic ulcers, after DAP therapy. Whole-genome sequencing analysis revealed that the isolate had a loss-of-function point mutation within liaX encoding DAP-sensing surface protein, which inhibits the LiaFSR systems and cell membrane remodeling. This is the first case report of a clinical DAP resistant E. faecalis with a mutation in liaX.

Keywords: Daptomycin; Enterococcus faecalis; LiaX; Whole-genome sequencing.

Publication types

Case Reports

MeSH terms

Anti-Bacterial Agents / pharmacology
Anti-Bacterial Agents / therapeutic use
Bacterial Proteins / genetics
Cell Membrane
Daptomycin* / pharmacology
Daptomycin* / therapeutic use
Drug Resistance, Bacterial / genetics
Enterococcus faecalis / genetics
Humans
Membrane Proteins
Microbial Sensitivity Tests
Mutation / genetics

Substances

Anti-Bacterial Agents
Bacterial Proteins
Membrane Proteins
Daptomycin